| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rhizobium etli | TGCTGATGGTTCACCGCA | TCAGCTGCCACATGCCAT | 59.57 | 59.64 | 171 | 94 |
100.00%
|
25.00%
|
| Primer 2 | Rhizobium etli | CCGAAACGTCTGCTCCTGT | GGCGATCGGGTCCTTGAAA | 60.01 | 60.08 | 213 | 93 |
100.00%
|
25.00%
|
| Primer 3 | Rhizobium etli | AGCCTTTCCCGTTTCCTGG | TCCCTTGAGCAGCACCATG | 59.93 | 60.00 | 226 | 89 |
100.00%
|
25.00%
|
| Primer 4 | Rhizobium etli | TCGAACGCGATATCGACCG | ACCCAAAGCACATCCGCA | 60.08 | 59.88 | 262 | 85 |
100.00%
|
25.00%
|
| Primer 5 | Rhizobium etli | TACGGTCGGGAAGCCATTG | AACGTCGATGATGCCCTGG | 59.78 | 60.15 | 198 | 84 |
100.00%
|
25.00%
|
| Primer 6 | Rhizobium etli | AATGATGCCGACGCTGCT | ATCGACATCGGGATTCGGC | 60.12 | 60.01 | 175 | 84 |
100.00%
|
25.00%
|
| Primer 7 | Rhizobium etli | TCATTCCGGTGCCGATCAC | AGAAGCTCACCGTCTGCAC | 60.15 | 60.01 | 276 | 81 |
100.00%
|
25.00%
|
| Primer 8 | Rhizobium etli | TTCGTTTCGTCGACCGCA | ACCAGCCGATGACGTTGA | 59.97 | 58.94 | 234 | 76 |
100.00%
|
25.00%
|
| Primer 9 | Rhizobium etli | TTCGTTTCGTCGACCGCA | GACCAGCCGATGACGTTGA | 59.97 | 60.08 | 235 | 76 |
100.00%
|
25.00%
|
| Primer 10 | Rhizobium etli | TGCGCATCGTCTCGGAAA | GGGTCGAATTCGGTGCTGA | 59.74 | 60.08 | 181 | 76 |
100.00%
|
25.00%
|